MICROBIOLOGY 301

PRINCIPLES OF MICROBIOLOGY
FALL, 2008

LECTURE: Lawson Hall, Room 151, 9:00 a.m. MWF
LABORATORY: Life Science II, Room 113 or 119

Section	Day	Time	Room No.
1	Monday	10:00-11:50 a.m.	119
	Wednesday	10:00 a.m.	119
2	Monday	10:00-11:50 a.m.	113
	Wednesday	10:00 a.m.	113
3	Monday	12:00-1:50 p.m.	113
	Wednesday	12:00 p.m.	113
4	Monday	12:00-1:50 p.m.	119
	Wednesday	12:00 p.m.	119
5	Tuesday	9:00-10:50 a.m.	113
	Thursday	9:00 a.m.	113
6	Tuesday	10:00-11:50 a.m.	119
	Thursday	10:00 a.m.	119
7	Tuesday	11:00 a.m.-12:50 p.m.	113
	Thursday	11:00 a.m.	113
8	Tuesday	2:00-3:50 p.m.	113
	Thursday	2:00 p.m.	113

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PROFESSORS	OFFICES	OFFICE HOURS
Dr. Michael T. Madigan	Life Science II, Room 187	T, R 2:00-5:00 p.m.
e-mail:	(or Lab, 109)	(or by appointment)
Phone: 453-5130
Dr. John M. Martinko	Life Science II, Room 126	M, W 2:00-5:00 p.m.
e-mail:	(or Lab 127)	(or by appointment)
Phone: 453-8116
TEACHING ASSISTANTS	SECTION(S)	OFFICES
Michelle Galeas	1	LSIII Rm 1032
(453-3820)
Angela Ko	8	LSIII Rm 1032
(453-3820)
Jamila Saidou	2 and 7	LSII Rm 127
(453-3721)
Samantha Sellers	3 and 6	LSII Rm 112
(453-4797)
George Tregoning	4 and 5	LSII Rm 112
(453-4797)

Please take advantage of the people that are here to help you in MICR 301. If you need help with either lecture or laboratory material, don't wait until the day before an exam or quiz to see your instructors; get things straightened out early. TAs will post their office hours and give you their e-mail addresses during the first week of class. For help in MICR 301, feel free to see your own TA or any of the other TAs, in addition to your professors. LABORATORIES BEGIN PERIOD 2 OF WEEK 1. During Labor Day week (week of Sept. 1) there will be some inconvenience for those in M/W labs, but if you bear with us, it will work out. During Veteran's Day week (week of Nov. 10) T/R labs will be affected in a similar way.

COURSE OBJECTIVES:

Lecture: To develop a fundamental understanding of the basic principles of microbiology. Students will develop a working understanding of the structure, growth, nutrition, metabolism, genetics and diversity of prokaryotes, and become familiar with medical, agricultural, and some other applied aspects of the field of microbiology.

Laboratory: To acquire the basic laboratory skills of microbiology. At the end of the course students will be able to: (1) handle and grow pure cultures of microorganisms safely; (2) perform manipulations involved in aseptic technique, diluting, pipetting, and enumerating microorganisms; (3) use a light microscope correctly; (4) sample and isolate bacteria from natural environments, including the human body, (5) perform and interpret basic immunological assays; and (6) accurately record, interpret, and present microbiological data in written form.

REQUIRED BOOKS:

Lecture: BROCK BIOLOGY OF MICROORGANISMS, 12th edition, 2009, Michael T. Madigan, John M. Martinko, Paul V. Dunlap, and David P. Clark. Benjamin Cummings/Pearson (available at the University, 710, and Saluki bookstores). Purchase EITHER the hardcover OR the ala'carte edition, not both. Assigned readings are listed by section number (see pages 6-9 of the syllabus). Readings and course materials are subject to change, and any changes will be announced in class.

Lab: Laboratory Manual for MICR 301: Available at The Printing Plant, 606 South Illinois Avenue. Some laboratory supplies (lab coat or apron, Sharpie marking pen, microscope slides, lens paper and notebooks) must also be purchased (available in all of the bookstores). In addition, a book entitled A Photographic Atlas to the Microbiology Laboratory is available in the bookstores; you may find this helpful for laboratory work but it is not required.

Web Sites:Two web sites will help you in MICR 301. The MICR 301 site can be found at:http://www.micro.siu.edu/micr301/. At this site you will find this syllabus, exam study questions, and links to other websites. In addition, your textbook supports a web site at: http://www.aw-bc.com/microplace/. This site offers study questions, interesting links, and current readings, some of which were used as sources in the preparation of your textbook. Check out both of the websites; they will help you learn the material and prepare for examinations.

GRADES: Total points for MICR 301: 590. Of the 590 possible points 400 (68%) are allotted to LECTURE and 190 (32%) to the LABORATORY. The grade and point distributions are as follows:

Lecture: Three hourly exams (100 points each) will be given during the semester (see LECTURE SCHEDULE for dates) along with a comprehensive final exam (200 points). The final exam can count for as much as half or as little as one quarter of your final lecture exam grade. How? Your final exam score will be divided in half to give two "hour-exam equivalents". Along with your three hour-exam scores, this will give you a total of five exam scores. The top FOUR of these FIVE scores will be summed to yield your lecture exam points (maximum lecture points = 400). BECAUSE THIS SYSTEM ALLOWS FOR YOU TO DROP AN HOURLY EXAM IF YOU WISH TO, NO MAKEUP EXAMS WILL BE GIVEN. If you miss an hourly exam, it is the one that will be dropped. All exams will be objective in nature (multiple-choice, true-false, matching) and will cover material from both the lecture and the assigned readings. You are responsible for assigned readings whether or not the material is covered in lecture. Any bonus points that show up on exams or quizzes DO NOT increase the number of points needed to achieve a particular grade. On exam days:

1. Be on time; set two alarm clocks if need be and if you drive to class, check your car the day before.
2. After the first student has finished the exam and left the classroom, exams will no longer be given out to latecomers.
3. Do not wear a hat with a brim (that is, a "baseball cap") on exam days.
4. Answer sheets will be machine scored. Thus, bring a sharpened #2 pencil and an eraser. Do not use ink on scantron sheets.
5. Instructors will seat you as you come into the auditorium. If you seat yourself, the instructors may reseat you. As you enter the classroom, please move to the front as far as possible. You should be seated with one empty desk between you and a classmate if possible. Use only a single desk to do your work.
6. No resource materials may be open or operated during the test. This includes books, notebooks, dictionaries (print or electronic), cell phones, computers, PDAs, BlackBerries, or any other print materials or electronic devices. Non-programmable calculators may be used.
7. Once an exam has started, you may not leave your seat without permission from an instructor until you have finished the exam.
8. It goes without saying that you will be academically honest and do your own work. Cheating will be dealt with according to SIU academic dishonest policies.

Lab:

Your attendance is fundamental to meeting the objectives of the lab. For the lab to be meaningful and for you to do well in lab, you must (1) be well prepared before you go to lab, and then (2) attend every lab period. Attendance in laboratory will be recorded. If you miss more than two lab periods (this is more than a full week of class), you will lose 40 lab points. Three tardies (you are tardy if you are more than 5 minutes late for class) equals one absence. There are no "make-up" labs in MICR 301. Because new materials are only available the first period of each week's labs, the labs are run on the stated schedule. If you miss a lab, you will have to get together with your partner and cover things in your notebook as best you can. Also, remember that quizzes are given at the beginning of lab periods; if you are tardy, you will miss the quiz. How can you do well in lab? It's actually very simple:

(1) Attend and be on time for every lab, (2) take the laboratory exercises and your notebook seriously, (3) be prepared for quizzes, and (4) follow both written and oral directions precisely. Your lab notebook is the written record of your laboratory experience. Your notebook will be collected three times during the semester and graded from 0-20 points each time (see details in your lab manual). (5) Follow the protocol in the laboratory manual and do the laboratory manipulations as instructed. Ten (10) technique points will be given by your lab instructor based on their assessment of how well you are prepared for lab and follow directions and whether or not you do the required manipulations ALL THE TIME. If you are lazy or careless in your technique, expect to lose both technique points and points in the lab practical exam. During the last week of the semester a laboratory practical exam will be given. This exam is designed to test key microbiological skills (dilutions, plating, aseptic technique, etc.) you will develop by doing the weekly laboratory exercises (see the last two pages of your lab manual for lab practical exam details). Fifteen point quizzes will be given periodically in lab throughout the semester. Quizzes will be announced the period before they are given. A total of four 15-point quizzes will be given throughout the semester but only the top three scores will count. The final quiz (20 points) will be comprehensive and given during the laboratory practical exam. NO MAKEUP QUIZZES OR LAB PRACTICAL EXAM WILL BE GIVEN.

MID-TERM STANDING: To calculate your approximate grade at mid-term, simply convert your lecture exam score and lab quizzes and notebook score to percentages and estimate your grade from the percentages given below under FINAL COURSE GRADE. Remember, however, that your lecture score is 68% of your final grade and your lab score is 32% of your final score. So, multiply your lecture exam average by 0.68 and your lab scores by 0.32, add the two numbers together and calculate your grade. Grade recording sheets can be found on the last page of this syllabus.

FINAL COURSE GRADE:

Of the 590 total points possible in MICR 301, 548 points (93%) are required to receive a grade of "A". To earn a "B", 483 points (82%) are required; to earn a "C", 418 points (71%) are required; and to pass the course, a student must end up with at least 354 points (60%). In addition, no student will pass the course, no matter how many points he/she has accumulated, without passing at least one exam (a passing score is defined as 60%).

TIPS FOR DOING WELL IN MICR 301:

1. Attend class, take notes, and study with your classmates. Nothing substitutes for being in class, listening, taking your own notes, and asking questions. Many years of teaching this course have shown that students that get the best grades in MICR 301 are the ones that come to class regularly. Class is where all of those subtle little things, like what is REALLY important, are revealed. Also, during class instructors will emphasize important concepts and illustrate them with figures and tables from the text; it is important that you note which text figures and tables are shown (see next paragraph on this). Also, read the assigned text readings BEFORE you come to class so that the material rings bells when it is presented. As you sit in lecture, take careful notes-writing is a form of learning. Unlike some other classes you are in or have been in, in MICR 301 it is critical that you take good notes during class; your notes are your lifeline to success on exams. Finally, if at all possible, study with a classmate. After you've "heard it" and "written it", "say it". Explaining concepts to a classmate and vice-versa will strengthen your learning experience.
2. Study on a continuous basis, not on a crisis basis. There is no substitute for starting your studying early. Review of lecture material as soon as possible after the lecture is the best way to study for MICR 301. This means that the evening following class (if not before) you should sit down and go through your notes in detail, highlighting the important points and filling in any gaps. Think about the lecture while you are doing this; what points were emphasized or seemed most important? Then re-read the text passages relating to the lecture while you have your notes in front of you; pay particular attention to what you perceived as key points and to figures from the text that were presented in class (hint: you will see some of these figures again in exam questions). If you study two weeks (or even two days) following a lecture instead of the day of a lecture, you will not achieve the learning benefit of immediate reinforcement. Staying on top of the material will make it fairly easy to prepare come exam time; your notes will be clear and sufficiently detailed to do well on the examination. In addition to this, careful review of the MiniReviews within each chapter of your text and answering the Study Questions at the end of each chapter will also help prepare you for lecture examinations.

¹Dr. Madigan will give lectures 1-15 and 17-27; Dr. Martinko will give lectures 16 and 28-41.

²Reading assignments are from Brock Biology of Microorganisms, 12th edition, Benjamin Cummings/Pearson 2009. Numbers (4.2, 6.3, etc.) refer to section numbers within a chapter. Instructors expect that students will have looked over the assigned readings before class. After lecture, a thorough reading of the material should help solidify your understanding (see "Tips for Studying MICR 301" on page 5). Syllabus readings and content are subject to changes announced in class.

³For lectures 25 and 26, material in Chapters 15-17 relevant to material discussed in class can be read for enrichment but is not required. Material from lectures 24-27 will be covered on the final exam.

Emergency Procedures