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The types of exam questions will be a variety of fill in the blank, matching, multiple choice, True/False and labeling/drawing diagrams.
Examples of the types of questions asked on MCIR 421 exams.
1. The ______________________________________________ involves the use of 4096 hexameric nucleotides for production of DNA probes.
2. Which two components would be present in a molecule of DNA? _____ and _____ .
A. glucose B. deoxyribose C. sulfate D. uracil E. thymine
3. Match the following: A. cDNA, B. mRNA, C. tRNA, D. rRNA
_____ Can be attached to an amino acid
_____ Product of transcription
_____ A component of ribosomes
_____ Synthesized prior to cloning a eukaryotic gene
4. What is the correct chronological order for the events listed below?
1st _____; 2nd _____; 3rd _____; 4th _____ . A. the structure of DNA was determined B. DNA was found to be the genetic material C. restriction enzymes were used to produce recombinant DNA D. the complete nucleotide sequence of the Haemophilus influenzae genome was determined
5. True or False in relation to the polymerase chain reaction.
______ 75OC is the temperature at which the primers hybridize to the target DNA
_____ Each cycle doubles the amount of the target DNA
_____ Taq is a DNA polymerase that is denatured at 95OC
_____ The forward primer hybridizes to the 5' flanking end of the DNA to be amplified
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