Department of Zoology
Southern Illinois University at Carbondale
ZOOL 409, Lab Week 10
Lab notes offer a preview of upcoming labs and links to more information about tissues examined during lab.
- Week 10 lecture notes
- SYLLABUS (including links to all note pages)
- 409 Homepage (index of course resources)
- Dr. King's School of Medicine histology page
- Slide summary
TUESDAY
Primary objective:Examine the respiratory tract.
- Slide 04, 06, 85, -- trachea. Note ciliated pseudostratified columnar epithelium (look for goblet cells), cartilage. You may find glands beneath the epithelium, and there may be smooth muscle between the ends of the cartilage rings.
- Slides 51, 71 -- lung. Note arrangement of alveoli. See (or imagine) the tissue elements (simple squamous epithelium, capillaries) comprising the alveolar wall. Look for "dust cells" (alveolar macrophages, with ingested particulate matter), and other evidence of dirt or soot from inhaled air. Look for bronchi and bronchioles with associated smooth muscle and blood vessels (veins and arteries of pulmonary circulation have more delicate walls than those of systemic circulation).
THURSDAY
Primary objective:Begin examination of kidney (to be continued next week).
- Slide 02 (also slide from Ms. Doran) -- kidney. There's a LOT going on, histologically, in the kidney. Begin by finding the basic regions.
- Capsule -- the outermost layer of connective tissue, enveloping the kidney.
- Cortex -- the outer region of kidney, consisting of convoluted tubules with scattered renal corpuscles.
- Medulla -- the deeper region of kidney, consisting of parallel tubules comprising loops of Henle and collecting ducts.
- Pelvis -- the "drain" of the kidney, which funnels urine from many tubules (collecting ducts) into the ureter. Transitional epithelium marks the beginning of the ureter. Fat and large blood vessels may be found in the pelvis.
- As you look for fine details, keep in mind how all of the details are organized into nephrons.
- In each renal corpuscle, distinguish the glomerulus from Bowman's capsule.
- Try to visualize Bowman's space and glomerular capillaries. Try to visualize (or imagine) three kinds of cells in the glomerulus:
- Look for corpuscles cut in such a way that you can distinguish:
- the vascular pole (where the glomerulus is attached);
- the urinary pole (where the urinary space is drained by the proximal convoluted tubule).
- In the renal cortex, try to distinguish proximal from distal tubules. (Proximal tubules are generally a bit larger in outside diameter, with larger, more eosinophilic cells. And because each proximal convoluted tubules is longer than the associated distal convoluted tubules, proximal tubules appear to be more plentiful in the cortex.)
- In the medulla, distinguish loops of Henle from collecting ducts. (Collecting ducts are lined by pale cuboidal cells with distinct boundaries between cells. Thick segments of the loop of Henle are lined by more eosinophilic cells with indistinct boundaries. Thin segments of the loops are lined by squamous cells with bulging nuclei.)
- Also note (or imagine) renal stroma (especially peritubular capillaries and vasa recta).
- Slide 08, 94 -- bladder and ureter. Note transitional epithelium, smooth muscle, connective tissue.
Complete slide list:
01, 02, 03,
04, 05, 06,
07, 08, 09,
10, 11, 12,
13, 14, 15,
16, 17, 18,
19, 20, 21,
22, 23, 24,
25, 26, 27,
28, 29, 30,
31, 32, 33,
34, 35, 36,
37, 38, 39,
40, 41, 42,
43, 44, 45,
46, 47, 48,
49, 50, 51,
52, 53, 54,
55, 56, 57,
58, 59, 60,
61, 62, 63,
64, 65, 66,
67, 68, 69,
70, 71, 72,
73, 74, 75,
76, 77, 78,
79, 80, 81,
82, 83, 84,
85, 86, 87,
88, 89, 90,
91, 92, 93,
94, 95, 96,
97, 98, 99,
100
Comments and questions: dgking@siu.edu
Department of Zoology e-mail: zoology@zoology.siu.edu
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