Department of Zoology
Southern Illinois University at Carbondale

ZOOL 409, Lab Week 3

Lab notes offer a preview of upcoming labs and links to more information about tissues examined during lab.

• Week 3 lecture notes
• SYLLABUS (including links to all note pages)
• 409 Homepage (index of course resources)
• Dr. King's School of Medicine histology page
• Slide summary

TUESDAY
Primary objective:  Recognize the common cellular and extracellular components of connective tissue, and distinguish several varieties of connective tissue.

[Secondary objective:  Review epithelial tissue as it appears.]

Find ordinary connective tissue in several (as many as possible) of the following organs. 

• Notice whether each occurrence is:
  • relatively dense, with mostly collagen fibers,
  • relatively loose, with more ground substance (clear area),
  • relatively cellular, with lots of lymphocytes (small round nuclei),
  • adipose (with lots of adipocytes)
• Notice presence of blood vessels and nerves.

• In skin, look for connective tissue of dermis immediately beneath the stratified squamous epithelium.  Distinguish dermis from deeper subcutaneous adipose (hypodermis).
  • skin -- slides 10, 43, 89, 92.
     
• In other organs with stratified squamous or transitional epithelium, look for CT deep to the epithelium.  Try to distinguish CT from muscle.
  • esophagus -- slides 06, 07, 68, 85.  (Muscle forms discrete layers.)
  • tongue -- slide 46.  (Skeletal muscle comprises the bulk of the organ.)
  • vagina -- slide 56.  (Smooth muscle is interspersed with the CT.)
  • bladder -- slide 08.  (Smooth muscle forms discrete layers.)
  • ureter -- slide 94.  (This slide shows a good example of adipose CT.)
       
    
• In hollow organs with simple columnar epithelium, distinguish lamina propria (CT with numerous lymphocytes, immediately beneath the epithelium) from the underlying submucosa (more fibrous, fewer cells).  Smooth muscle forms a discrete layer.
  • colon -- slides 09, 38, 48.
  • intestine -- slides 03, 05, 37, 47, 61, 70.
  • stomach -- slides 40, 41, 68.
     
• In "solid" (parenchymal) organs, try to distinguish CT of stroma, interspersed in thin or thick strands between epithelial parenchyma.
  • kidney -- slide 02.  (Very thin stroma, nice peri-renal adipose CT.)
  • salivary gland -- slide 42.  (Very thin stroma between acini [clusters of secretory cells]; thicker strands between lobules.)
  • pancreas -- 65, 70, 84.  (Very thin stroma between acini [clusters of secretory cells]; thicker strands between lobules.)
    
  • mammary gland -- slides 72, 73.  (Relatively thick stroma between epithelial tubules.)
     
• In the slide of blood vessels, look for CT between the vessels.
  • blood vessels -- slide 30. 

THURSDAY Objective:  Visit a working histology laboratory and learn how specimens are prepared.  

Meet in Room 2055 of Life Science 3 (immediately south of LSII).

Complete slide list:
01, 02, 03, 04, 05, 06, 07, 08, 09, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100

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