Department of Zoology
Southern Illinois University at Carbondale
ZOOL 409, Lab Week 9
Lab notes offer a preview of upcoming labs and links to more information about tissues examined during lab.
- Week 9 lecture notes
- SYLLABUS (including links to all note pages)
- 409 Homepage (index of course resources)
- Dr. King's School of Medicine histology page
- Slide summary
TUESDAY
Primary objective:
- Continue your examination of the digestive tract (several regions), concentrating now on glands and specialized secretory cell types.
- Slide 42, 46, 91 -- salivary glands (distinguish serous cells, mucous cells, and ducts)
- Slides 85, 68 (also, in some boxes, 06, 07), esophagus -- (submucosal glands may or may not be present).
- Slides 40, 41, 68 -- stomach
The stomach is notable for its prominent mucosal glands. Note distinct surface mucous cells in all regions, extending into gastric pits. Then note that the mucosal glands differ in different regions of the stomach. Distinguish fundic glands, with their plentiful chief cells and parietal cells, from the mucous glands of cardiac and pyloric stomach.)
- Slides 03, 05, 24, 37, 47, 70 -- small intestine (distinguish goblet cells from absorptive cells in the surface of the villi; see whether you can distinguish Paneth cells at the bottoms of the crypts, and note Brunner's glands in the submucosa of the duodenum.)
- Slides 65, 70, 84 -- pancreas (note serous acini, ducts, and islets)
THURSDAY
Primary objective:
- Examine liver (and continue your examination of the digestive tract).
- Slide 49 -- Pig liver has each hepatic lobule neatly outlined by connective tissue. Note hepatic lobules, portal areas (each with portal vein, hepatic artery, and bile duct), hepatic cords, sinusoids, and central veins. Understand the relationships among hepatocytes, sinusoids, and bile canaliculi. Try to see sinusoidal endothelium, space of Disse, and Kupffer cells.
Human liver is quite similar, except without conspicuous septa separating the lobules. Microscopically the human liver looks like a vast field of hepatocytes interrupted only occasionally by portal areas.
Slide 49 is stained with trichrome, so collagenis blue (highlighting the "frame"around each lobule. But unfortunately nuclei haven't stained well, so you can't use this slide -- at least not easily -- to see the various cells which comprise liver.
- Slide 50 -- Frog liver, stained with PAS to highlight glycogen storage.
Frog liver differs in its organization from human / pig liver, and also has very large melanin-storing phagocytes. Also note that the blood cells in the sinusoids all contain nuclei (i.e., the nucleated red-blood-cells might be mistaken for inflammatory infiltrate).
- Slide 64 -- Liver stained for mitochondria. This is not an especially useful specimen, although it does display mitochondria.
- Slide 95 -- Liver with cirrhosis. Look for the same organizational features as in pig liver. Then note that connective tissue between lobules is variously thick and inflamed.
The term cirrhosis refers to scarring of the liver, in which collagen replaces damaged liver parenchyma. For more, see liver pathology.
Complete slide list:
01, 02, 03,
04, 05, 06,
07, 08, 09,
10, 11, 12,
13, 14, 15,
16, 17, 18,
19, 20, 21,
22, 23, 24,
25, 26, 27,
28, 29, 30,
31, 32, 33,
34, 35, 36,
37, 38, 39,
40, 41, 42,
43, 44, 45,
46, 47, 48,
49, 50, 51,
52, 53, 54,
55, 56, 57,
58, 59, 60,
61, 62, 63,
64, 65, 66,
67, 68, 69,
70, 71, 72,
73, 74, 75,
76, 77, 78,
79, 80, 81,
82, 83, 84,
85, 86, 87,
88, 89, 90,
91, 92, 93,
94, 95, 96,
97, 98, 99,
100
Comments and questions: dgking@siu.edu
Department of Zoology e-mail: zoology@zoology.siu.edu
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